Image Processing and Analysis of the Scratch Assay by Eyad GadImage Processing and Analysis of the Scratch Assay by Eyad Gad

Image Processing and Analysis of the Scratch Assay

Eyad Gad

Eyad Gad

Scratch Area - Origin
Scratch Area - Grayscale
Scratch Area - binary
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Posted Jul 2, 2025

Scratch assay uses time-lapse images to track cell migration. Segmentation with entropy filter & Otsu in Python shows area shrinking over time.